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Shtisel 101: Animal Experimentation
(ה) כִּֽי־תִרְאֶ֞ה חֲמ֣וֹר שֹׂנַאֲךָ֗ רֹבֵץ֙ תַּ֣חַת מַשָּׂא֔וֹ וְחָדַלְתָּ֖ מֵעֲזֹ֣ב ל֑וֹ עָזֹ֥ב תַּעֲזֹ֖ב עִמּֽוֹ׃ {ס}
(5) When you see the ass of your enemy lying under its burden and would refrain from raising it, you must nevertheless raise it with him.
(ד) לֹא־תַחְסֹ֥ם שׁ֖וֹר בְּדִישֽׁוֹ׃ {ס}
(4) You shall not muzzle an ox while it is threshing.
What is the goal in prohibiting the muzzling of the ox?

(א) שלא לחסום בהמה בשעת מלאכתה -....

(ב) משרשי המצוה. ללמד עצמנו להיות נפשנו נפש יפה בוחרת הישר ומדבקת בו ורודפת אחר החסד והחמלה, ובהרגילנו אותה על זה אף על הבהמות שלא נבראו רק לשמשנו, לחוס עליהן לחלק להן חלק מיגיעת בשרן תקח לה הנפש דרכה בהרגל זה, להטיב אל בני אדם ולשמור אותם מהעביר עליהם הדרך בשום דבר שראוי להם, ולשלם שכרם ככל אשר יעשו טוב, ולהשביעם מאשר יגעו בו, וזה הדרך ראוי ילכו בה עם הקדש הנבחר.

(1) To not muzzle an animal at the time of its work:...

(2) It is from the roots of the commandment [that it is] to teach ourselves that our souls be a good soul that chooses what is right and clings to it and pursues kindness and mercy. And in our accustoming it to this - even with animals that were only created to serve us, to be concerned for them to distribute to them a portion of the toil of their flesh - the soul will take for itself the way of this habit; to do good to people and to guard from taking access away from them for anything that is appropriate for them, and to repay their reward according to all the good that they do and to satiate them with that upon which they toiled. And it is fitting for the holy chosen nation to follow this way.

(י) יוֹדֵ֣עַ צַ֭דִּיק נֶ֣פֶשׁ בְּהֶמְתּ֑וֹ וְֽרַחֲמֵ֥י רְ֝שָׁעִ֗ים אַכְזָרִֽי׃
(10) A righteous man knows the needs of his beast,
But the compassion of the wicked is cruelty.
ע"י מעשה באו מאי היא דההוא עגלא דהוו קא ממטו ליה לשחיטה אזל תליא לרישיה בכנפיה דרבי וקא בכי אמר ליה זיל לכך נוצרת אמרי הואיל ולא קא מרחם ליתו עליה יסורין וע"י מעשה הלכו יומא חד הוה קא כנשא אמתיה דרבי ביתא הוה שדיא בני כרכושתא וקא כנשא להו אמר לה שבקינהו כתיב (תהלים קמה, ט) ורחמיו על כל מעשיו אמרי הואיל ומרחם נרחם עליה
The Gemara stated that Rabbi Yehuda HaNasi’s suffering came upon him due to an incident. What was that incident that led to his suffering? The Gemara answers that there was a certain calf that was being led to slaughter. The calf went and hung its head on the corner of Rabbi Yehuda HaNasi’s garment and was weeping. Rabbi Yehuda HaNasi said to it: Go, as you were created for this purpose. It was said in Heaven: Since he was not compassionate toward the calf, let afflictions come upon him. The Gemara explains the statement: And left him due to another incident. One day, the maidservant of Rabbi Yehuda HaNasi was sweeping his house. There were young weasels [karkushta] lying about, and she was in the process of sweeping them out. Rabbi Yehuda HaNasi said to her: Let them be, as it is written: “The Lord is good to all; and His mercies are over all His works” (Psalms 145:9). They said in Heaven: Since he was compassionate, we shall be compassionate on him, and he was relieved of his suffering.
Halachic Conclusion

... כל דבר הצריך לרפואה או לשאר דברים לית ביה משום איסור צער בעלי חיים (א"ו הארוך סימן נ"ט) ולכן מותר למרוט נוצות מאוזות חיות וליכא למיחש משום צער בעלי חיים (מהרא"י סי' ק"ה) ומ"מ העולם נמנעים דהוי אכזריות:

(14) ...Any [action] needed for healing or other reasons, there is no prohibition of "causing pain to animals" (Issur V'Heter Extended 59). And therefore it is permitted to pluck the feathers of wild geese, and there is no potential problem of "causing pain to animals" (Mahar"i 105). Nevertheless, the world withholds from it because of its cruelty.